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Oligo Synthesis

Oligo Synthesis : CEPs

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Pac-A-CE Phosphoramidite

Pac-A-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3000-02Pac-A-CE Phosphoramidite 0.25g £68.00 Quantity Add to Order
10-3000-05Pac-A-CE Phosphoramidite 0.5g £136.00 Quantity Add to Order
10-3000-10Pac-A-CE Phosphoramidite 1.0g £250.00 Quantity Add to Order

Description

Pac-A-CE Phosphoramidit

 

Structure

Catalog Number: 10-3000-xx

Description: Pac-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-phenoxyacetyl-Adenosine,2'-O-TBDMS-3'-
[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C54H68N7O9PSi M.W.: 1018.23 F.W.: 329.21

Diluent: Anhydrous Acetonitrile
Coupling: 12 minutes. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: RNA Deprotection-see bulletin Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

TBDMS-PROTECTED RNA PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

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Protocols

Material Safety Data Sheet

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3000-02 0.25grams .25grams 2.46 68.67 41.2 25.75 18.73 13.73 3.43
10-3000-05 0.5grams .5grams 4.91 150.33 90.2 56.38 41 30.07 7.52
10-3000-10 1.0gram 1grams 9.82 314 188.4 117.75 85.64 62.8 15.7
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3000-02 0.25grams .25grams 3.66 .07 N/A N/A 13.92 N/A
10-3000-05 0.5grams .5grams 7.33 .07 N/A N/A 29.21 N/A
10-3000-10 1.0gram 1grams 14.66 .07 N/A N/A 59.75 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3000-02 0.25grams .25grams 3.66 .07 N/A 11.03 10.36

10-3000-05 0.5grams .5grams 7.33 .07 N/A 22.87 21.48

10-3000-10 1.0gram 1grams 14.66 .07 N/A 46.52 43.7

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Related products

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