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Includes MasterAmp™ PCR Enhancer (with betaine) for improved PCR
MasterAmp™ Tth DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus thermophilus. It has optimal synthetic activity at temperatures above 70deg;C and can be used at temperatures up to 95°C, making it useful for applications such as PCR. The enzyme also has thermostable reverse transcriptase activity, which enables synthesis of cDNA from RNA templates. The high reaction temperatures possible with Tth DNA Polymerase minimize problems associated with nonspecific priming and template secondary structure. MasterAmp™ Tth DNA Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA. A 10X solution of MasterAmp PCR Enhancer (with betaine) is provided with the enzyme. The MasterAmp™ PCR Enhancement Technology* substantially increases both the probability of obtaining the desired amplification product and the reaction-to-reaction consistency of PCR.
Unit Definition
One unit of MasterAmp™ Tth DNA Polymerase converts 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions using a DNA template.
Storage Buffer
50% glycerol containing 50mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 0.5% Tween 20, 0.5%NP-40, and 1 mM DTT.
Quality Control
MasterAmp™ Tth DNA Polymerase is tested for the absence of detectable, contaminating, nonspecific DNA exo- and endonuclease and RNase activities, and is functionally tested in PCR.
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