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DNA & RNA Sequencing

DNA & RNA Sequencing : Next Generation Sample Prep

rRNA removal from RNA preparations for Next Generation Sequencing

DNA Normalizer v3

DNA Normalizer is based on Aline’s patented magnetic bead technology. This technology allows a pre-determined amount of DNA (output) to be isolated from variable DNA input and the output can be adjusted based on the end users’ needs.

Aline Biosciences

Catalogue No.DescriptionPack SizePriceQty
AL-N-4300-LAline DNA Normalizer v34x1000 preps POA Quantity Add to Order
AL-N-4300-MAline DNA Normalizer v31000 preps £740.00 Quantity Add to Order
AL-N-4300-SAline DNA Normalizer v3100 preps £143.00 Quantity Add to Order

Description

 

DNA Normalizer V3

 

Product Overview:

The patented DNA Normalizer Kit v3 is designed for an automated DNA library normalization process based on Aline’s patented magnetic bead technology. This technology allows a pre-determined amount of DNA (output), which can be adjusted to suit the end-users’ needs, to be isolated from variable DNA input. In addition, DNA normalization is accomplished during this purification process so that additional DNA quantification and dilution is not necessary. Time, labor, and reagent costs are greatly saved with Aline’s unique normalization purification system. All DNA fragments (in PCR amplification or enzymatic reactions) can be normalized and combined in one simple process for next-generation library construction.

Note: The DNA output following the standard protocol is about 100ng per reaction. However, the output can be adjusted by end-users if desired. The medium kit is for 1000 reactions.

 

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Key Features:

  • Excellent dynamic range, as wide as 7 fold difference in input DNA concentration
  • No more Picogreens; saves time and labor
  • Multiple simultaneous actions: Normalization, clean-up, and concentration
  • Consistent normalization result
  • Manual and automation-friendly
  • Yields double-stranded DNA
  • Suitable for exon capture as well and any other molecular engineering applications

 

Platforms:

  • Compatible with ALL next-generation sequencing platforms

 

New Advantages of DNA Normalizer v3 vs DNA Normalizer v2:

  • Can be used in both PCR product and fragmented genomic DNA normalization
  • Extra primer dimer/adapter removal reagent is available as an option
  • Extended shelf life: to 12 months
  • Input DNA quantity: only two-fold of targeted output DNA is needed

New Advantages – DNA Normalizer v3

  • Can be used in both genomic DNA and PCR/fragmented DNA normalization
  • higher primer dimer/adapter removal steps included in the kit as an option
  • Extended Shelf Life

Additional information

Small 100-400 rxns

Medium 1,000-4,000 rxns
Large 2,000-8,000 rxns
For Bulk Contact us

Storage:

  • Store at 4°C upon arrival
  • Resuspend
  • PCRClean DX (optional) and DNA Normalizer V3 Beads well before using
  • DO NOT FREEZE

Stability:

  • 12 months if stored as specified

 

Disclaimer: For research use only. Not for use in diagnostic procedures.

 

 

 

 

 

 

 


If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

 

Datasheet - click here

 

Protocol - click here

 

SDS - click here

 

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200