Proteins tagged with the epitope sequence of the rho1D4 antibody can be purified with this affinity matrix and eluted with Rho1D4 peptide (available separately). Our high quality Rho1D4 resin for purification of membrane proteins is available in pre-packed affinity chromatography columns, ready to be used on automated liquid chromatography systems (e.g., FPLC). Our pre-packed columns are available in 2 sizes, 1 mL and 5 mL bed volumes. Cambio FPLC cartridges are also available empty, to be filled with your chromatography matrix of choice. For purification in batch or by manual chromatography please see our “Rho1D4 Agarose” or for dilute starting material and pulldowns check out Rho1D4 Magbeads.
Cartridge for low pressure chromatography (FPLC), packed with 1 ml Rho1D4 agarose
Cambio Rho1D4 Cartridges provide:
- ideal solution for membrane protein research
- binding capacity of 3-4 mg protein per mL resin
- compatibility with common chromatography instruments
- gentle protein elution based on competitive binding
- excellent flow distribution and minimal void volume
Table1: Technical features of Cambio FPLC Cartridges
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Cartridge, 1 mL
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Cartridge, 5 mL
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Bed volume
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1 mL
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5 mL
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Dimensions (mm)
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6.2 X 50
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11 X 80
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Body material
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Polypropylene
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Acrylate
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Inlet
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10-32 UNF female thread
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10-31 UNF female thread
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Outlet
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10-32 UNF female thread
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10-32 UNF female thread
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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200