Description
RiboExTM is a complete kit with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. RiboExTM is based on the disruption of cells in guanidine salt / detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and it allows simultaneous processing of a large number of samples. RiboExTM can yield up to 10 ug / 1 mg tissue or up
to 30 ug / 1 x 106 cultured cells of highly purified total RNA. The resulting total RNA is suitable for the isolation of poly A RNA, northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RNase protection assays and other analytical procedures.
Features and Benefits
¦ Format : Monophase solution type
¦ Sample size : Up to 100 mg tissue / 1 ml RiboExTM
Up to 1 x 107 cells / 1 ml RiboExTM
¦ Preparation time : 50 ~ 65 minutes
¦ Typical yield : Up to 10 ug / 1 mg tissue
Up to 30 ug/ 1 x 106 cultured cells
¦ High purity : OD260/230 > 2.0, OD260/280 > 1.8
¦ Accurate and consistent yield from animal tissue, cultured cell line, plant, E. coli
and various biological samples
¦ Accurate and easy phase separation
¦ Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning,
real-time PCR, RNase protection assays and other analytical procedures
Procedure
Specification
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