Cambio - Excellence in Molecular Biology

Extraction & Purification - RNA

Extraction & Purification - RNA: Tissue/Cultures

RNA Isolation from tissues and cell cultures

Ribospin™ vRD (plus!)

Ribospin™ vRD provides a convenient method for isolation of RNA and DNA from cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples. Ribospin™ vRD procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA and DNA isolation, instead of conventional alcohol precipitation or phenol/chloroform extraction. Ribospin™ vRD buffer system provides the effective binding condition of RNA and DNA to glassfiber membrane ...

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Description

 Description

Ribospin™ vRD provides a convenient method for isolation of RNA and DNA from cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples. Ribospin™ vRD procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA and DNA isolation, instead of conventional alcohol precipitation or phenol/chloroform extraction. Ribospin™ vRD buffer system provides the effective binding condition of RNA and DNA to glassfiber membrane through mix with lysis and binding buffers. And then the impurities on the membrane are washed away by two different wash buffers. At last, pure RNA and DNA are eluted by nuclease-free water. Whole procedure may take only 20 minutes and the eluate is suitable for PCR, RT-PCR or any downstream application without further manipulation. Ribospin™ vRD plus! kit offers carrier RNA for purification of nucleic acid from very small amounts of sample.

 

Features and Benefits

¦ Glassfiber membrane technology
¦ Sample size : up to 300 ?
¦ Preparation time : ~ 20 minutes
¦ Stable and consistent yield
¦ No phenol / chloroform extraction
¦ No ethanol precipitation
¦ Ready for use in PCR, RT-PCR, real-time PCR and other analytical procedures

 

Procedure

 

 

Specification

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Protocols

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References

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200