Cambio - Excellence in Molecular Biology

Cell culture contamination control

Cell culture contamination control: Misc Products

Venor® Quantification Standards

Venor® Quantification Standards for Mycoplasma Detection from Minerva

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQty
52-0071Pseudomonas aeruginosaPseudomonas aeruginosa £215.80 Quantity Add to Order
52-0083Escherichia colieach £215.80 Quantity Add to Order
52-0101Legionella pneumophila  £215.80 Quantity Add to Order
52-0112Mycoplasma orale  £215.80 Quantity Add to Order
52-0115Mycoplasma gallisepticumMycoplasma gallisepticum £215.80 Quantity Add to Order
52-0116Acholeplasma laidlawii  £215.80 Quantity Add to Order
52-0117Mycoplasma fermentans  £215.80 Quantity Add to Order
52-0119Mycoplasma pneumoniae  £215.80 Quantity Add to Order
52-0124Mycoplasma synoviaeMycoplasma synoviae £215.80 Quantity Add to Order
52-0129Mycoplasma argininiMycoplasma arginini £215.80 Quantity Add to Order
52-0130Mycoplasma hyorhinis  £215.80 Quantity Add to Order
52-0164Spiroplasma citriSpiroplasma citri £215.80 Quantity Add to Order
52-5571Bordetella pertussisBordetella pertussis £215.80 Quantity Add to Order

Venor® Quantification Standards

Venor® Quantification Standards for Mycoplasma Detection from Minerva

Minerva Biolabs

The quantification standard is genomic DNA extracted from low passage and defined mycoplasma or legionella cultivated in liquid medium according to the European Pharmacopoeia or in Hayflick's medium. The extraction of the DNA is done by column absorption methods and the DNA purified by phenol/chloroform extraction and ethanol precipitation. The concentration was determined photometrically and by real-time PCR. The DNA concentration was adjusted by dilution with DNA stabilizing buffer. Genomic DNA is used as a control template when DNA amplification tests by conventional PCR are performed (e.g. as contamination control in cell culturing). With the real-time PCR technology, this titrated DNA standard with a defined concentration of genome copies is used as calibrator to generate standard curves. Therefore the provided solution is diluted in a 10x dilution series with DNA-free water and used as a PCR sample. With the generated fluorescent data, the software provided with the real-time PCR instrument calculates a standard curve, which can be used to determine the DNA concentration of unknown samples.

Contents

1 vial (green cap) of Standard DNA, 100 µl, contains approx. 106 genomes/µl.
3 vials (white cap) with 1 ml of DNA free water.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Venor® Quantification Standards

Venor® Quantification Standards for Mycoplasma Detection from Minerva

Minerva Biolabs

Protocols

Legionella pneumophila
Mycoplasma synoviae
Mycoplasma orale
Acholeplasma laidlawii
Please note: all protocols off site are the responsibility of the products supplier
 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Venor® Quantification Standards

Venor® Quantification Standards for Mycoplasma Detection from Minerva

Minerva Biolabs

Dumke R. et al., (2017). Multi-center evaluation of one commercial and twelve in-house real-time PCR assays for detection of Mycoplasma pneumoniae. Diagnostic Microbiology & Infectious Disease, doi: 10.1016/j.diagmicrobio.2017.03.004.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Venor® Quantification Standards

Venor® Quantification Standards for Mycoplasma Detection from Minerva

Minerva Biolabs

Applications

These DNA-Standards allow for the performance of a control reaction for PCR using qPCR instrumentation and to create a standard curve for quantification experiments, i.e. with Venor®Mp Mycoplasma pneumoniae Diagnostic Kit for qPCR. The DNA standards can be used as a general control to confirm PCR system performance using a qPCR instrument or for the validation of detection limits in respect to conventional PCR tests.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200