| 5'-Dabsyl-dT-CE Phosphoramidite |
Catalog Number: 10-1532-xx
Description: 5'-Dabsyl-dT-CE Phosphoramidite
5'-Dabsyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite |
| Formula: C33H44N7O8PS |
M.W.: 729.78 |
F.W.: 591.53 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dGwith acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2days |
Dabcyl LABELLING
A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.
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