Cambio - Excellence in Molecular Biology

In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames MPF 98/100/1535/1537 Test Kits - semi-solid

The Ames MPF 98/100/1535/1537 assay comprises the histidine auxotrophic Salmonella tester strains and TA98 and TA1537 for detecting frameshift mutations,

Xenometrix AG

Description

The Ames MPF 98/100/1535/1537 assay comprises the histidine auxotrophic Salmonella tester strains and TA98 and TA1537 for detecting frameshift mutations, as well as TA100 and TA1535 which are reverted by base-pair substitutions and all media required to perform the assay. Kits including additional S9 and Positive Controls are also available.

Kit contents

(Depending on kit size)


1 or 10 Vials semisolid TA98
1 or 10 Vials semisolid TA100
1 or 10 Vials semisolid TA1535
1 or 10 Vials semisolid TA1537
1 or 10 Vials Ampicillin
Growth -, Exposure - and Reversion Indicator Media
All media and strains provided are sufficient to analyze at least 11 or 10 compounds,
triplicates, 6 concentrations, negative and positive controls, /- S9

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