Description
Glen UnySupport™ is a universal
support based on a conformationally preorganized molecule to accelerate
the dephosphorylation reaction. The N-phenyl version, developed at Isis
Pharmaceuticals as UnyLinker™, is available from several companies for
large scale oligonucleotide synthesis. Glen UnySupport™ is the N-methyl
version, which is preferred for high throughput oligonucleotide
synthesis since methylamine rather than aniline is formed on
deprotection. Higher angstrom universal supports are ideal for long
syntheses (>150 mer) and can be utilized with modifier
phosphoramidites for which 2000Å supports are not readily available.
Usage
- Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
- Deprotection:
Solution phase: Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at
65°C or Ammonium Hydroxide for 8 hours at 55°C. For sensitive minor
bases or dyes, Glen UnySupport may be eliminated with 50 mM Potassium
Carbonate in Methanol in 17 hours at room temperature or with
Tert-Butylamine/w
Intellectual Property
This product is covered by US Patent 7,202,264 owned by Ionis Pharmaceuticals, Inc..
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References
REFERENCE(S)| 1 A.P. Guzaev, and M.
Manoharan, J Am Chem Soc, 2003, 125, 2380- 2381.| 2 R.K. Kumar, A.P.
Guzaev, C. Rentel, and V.T. Ravikumar, Tetrahedron, 2006, 62, 4528.
ater 1:3 (v/v) for 4 hours at 60°C. Gas Phase:
Methylamine gas for 30 minutes at 65°C at 30 psi.
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