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Aptamers

Aptamers: Catalogue Aptamers

Important information

Do you know what modifications you will need for your aptamers?  see "modifications" section for details

BNP

Brain Natriuretic Peptide ATW0079

Base Pair Bio

Catalogue No.DescriptionPack SizePriceQty
ATW0079-100BNP10 AEU (100ug) POA Quantity Add to Order
ATW0079-25BNP2.5 AEU (25ug) POA Quantity Add to Order

Description

Brain Natriuretic Peptide, BNP-32, is a 32-amino acid peptide that is synthesized  within the ventricles of the heart. Natriuretiuc peptides regulate sodium and water balance, blood volume, and arterial pressure. BNP-32 is a biomarker for heart failure.

DataSheet

Target:   BNP (Brain Natriuretic Peptide)

Target Species:   human

Target Type:   peptide

Affinity Constant (Kd):   61.3 nM

Default 3' Linker (Spacer) is 10T. Please submit a custom quote request for other options.

Aptamer was selected at Base Pair Biotechnologies from a randomized 32-mer DNA library against the target protein.

 

Proprietary methods were then used to select the aptamer.

 

Aptamer folding instruction before use:

Once the aptamer is in its working concentration, it needs to be heated to 85-95 ºC for 5 minutes, and then cooled to room

Temperature (~15 minutes) before use.

Validation data by Microscale Thernophoresis (MST) analysis:

Buffer used for validation: 1X PBS, pH = 7.4, 1 mM MgCl2, 0.05% Tween

Average Kd: 61.3 ± 9.9 nM

 

Please choose your modification and add as an extra catalogue number:

ATWUNMOD - Unmodified

ATWAMINE - Amine modification

ATWBIOTIN - Biotin modification

ATWTHIOL - Thiol modification (extra charge)

ATWFLUOR - Fluor (all) modification (extra charge)

 

Custom aptamers can be developed; contact us for details


If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

DataSheet

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Please note that aptamers have much lower molecular weights than antibodies. Thus, there are typically five to fifteen times as many moles or molecules of aptamers present in a solution containing an equal mass. For example, 100 micrograms of an aptamer is equivalent to 0.5 to 1.5 mg of antibody, depending on the particular aptamer.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200