Description
The FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation, compatible with visualization of proteins separated by 1-D or 2-D SDS-PAGE. FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is normally low fluorescent but emits strong fluorescence (bright golden color) as bound to proteins. The staining procedure is a simple two-step protocol (fix and stain) that can be completed in as little as 30 minutes. Gels to be stained are fixed with ethanol/acetic acid solution prior to staining with FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) solution. A destain step is not recommended, but may be employed to reduce background, simply by agitating the gel in water for 1-5 minutes.
Fig. 1. Excitation and emission spectrum of FluoroStain Protein Fluorescent Staining Dye (Gold, 100×).
Gels stained with FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) may be directly visualized with a variety of different UV-based fluorescence imaging systems. The maximum emission wavelength of protein-bound FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is near 570 nm. FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) gives exceptional sensitivity and wide dynamic range for protein detection. The bound FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is easily removed from the protein by immersing the gel in sufficient water, thus it is well compatible with subsequent enzymatic digestion and mass spectrometry for proteomics applications. Stained gels may be stored in stain solution in the dark at 2-8°C; imaging sensitivity might be moderately enhanced after 4°C storage of the stained gel.
Fluorescence Characteristics
FluoroStain Protein Fluorescent Staining Dye (Gold, 100×) has its excitation peaks at 330 and 390 nm and emission maximum at 570 nm, making it compatible with UV-based imagers.
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