Description
ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the
purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/ug with ExfectionTM EF and to 10 EU/ug with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.
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Features and Benefits
¦ Spin column format based on glassfiber membrane
¦ Convenient clearing of lysate with EzClearTM filter (Midi)
¦ High plasmid recoveries with high purity
¦ Fast preparation time and simple procedure
¦ High transfection efficiency in most cell-lines
¦ No need of additional materials
¦ No use of organic solvents
Technical Information
| Features |
Plasmid LE mini |
Plasmid LE Midi |
| Format |
Column Type Qe with collection tube | Column Type E with collection tube |
| Recommended sample volume | 2~10 ml LB | 5~100 ml LB |
| Maximum sample volume | Up to 10 ml LB | Up to 100 ml LB |
| Lysate clearing | Centrifugation | EzClear™ Filter |
| Preparation time | under 30 minutes | under 50 minutes |
| Maximum loading volume | 800 μl
| 15 ml |
| Binding capacity | 30 μg | 300 μg |
| Endotoxin level | <10 EU/μg | <10 EU/μg
|
| Maximum Elution volume | 50 μl | 500 μl |
Technical Data
Transfection Efficiency
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