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Oligo Synthesis

Oligo Synthesis : CEPs

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dA-5'-CE Phosphoramidite & dC-5'-CE Phosphoramidite

3'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite 3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-0001-02dA-5'-CE Phosphoramidite 0.25g £64.00 Quantity Add to Order
10-0001-05dA-5'-CE Phosphoramidite 0.5g £123.00 Quantity Add to Order
10-0001-10dA-5'-CE Phosphoramidite 1.0g £236.00 Quantity Add to Order
10-0101-02dC-5'-CE Phosphoramidite 0.25g £64.00 Quantity Add to Order

Description

dA-5'-CE Phosphoramidite
dC-5'-CE Phosphoramidite

Structure

Catalog Number: 10-0001-xx

Description: dA-5'-CE Phosphoramidite

3'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine,5'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C47H52N7O7P M.W.: 857.95 F.W.: 313.21
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

5'-CE PHOSPHORAMIDITES

Glen Research 5'-CE (ß--cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies. These monomers are packaged in ABI-style vials (see note box).

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Protocols

Material Safety Data Sheet

Glen Report 6.1: 5' TO 3' SYNTHESIS

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-0001-02 0.25grams .25grams 2.91 83.67 50.2 31.38 22.82 16.73 4.18
10-0001-05 0.5grams .5grams 5.83 181 108.6 67.88 49.36 36.2 9.05
10-0001-10 1.0gram 1grams 11.66 375.33 225.2 140.75 102.36 75.07 18.77
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-0001-02 0.25grams .25grams 5 .058 93.6 58.5 42.55 5.85
10-0001-05 0.5grams .5grams 10 .058 193.6 121 88 12.1
10-0001-10 1.0gram 1grams 20 .058 393.6 246 178.91 24.6
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-0001-02 0.25grams .25grams 5 .058 95.2 59.5 43.27

10-0001-05 0.5grams .5grams 10 .058 195.2 122 88.73

10-0001-10 1.0gram 1grams 20 .058 395.2 247 179.64

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Related products

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