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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-OMe-C-CE Phosphoramidite

2'-OMe-C-CE Phosphoramidite

Glen Research

Description

2'-OMe-C-CE Phosphoramidite

Structure

Catalog Number: 10-3110-xx

Description: 2'-OMe-C-CE Phosphoramidite

5'-Dimethoxytrityl-2'-O-methyl-N-benzoyl-Cytidine, 3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C47H54N5O9P M.W.: 863.95 F.W.: 319.21
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 days

2’-Ome-rna phosphoramidites

Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

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Protocols

MSDS

Glen Report 6.2

Glen Report 6.1

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. 

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3110-02 0.25grams .25grams 2.89 83 49.8 31.13 22.64 16.6 4.15
10-3110-05 0.5grams .5grams 5.79 179.67 107.8 67.38 49 35.93 8.98
10-3110-10 1.0gram 1grams 11.57 372.33 223.4 139.63 101.55 74.47 18.62
10-3110-90 100µmoles .086grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3110-02 0.25grams .25grams 4.32 .07 80 50 36.36 5
10-3110-05 0.5grams .5grams 8.64 .07 166.4 104 75.64 10.4
10-3110-10 1.0gram 1grams 17.28 .07 339.2 212 154.18 21.2
10-3110-90 100µmoles .086grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3110-02 0.25grams .25grams 4.32 .07 81.6 51 37.09

10-3110-05 0.5grams .5grams 8.64 .07 168 105 76.36

10-3110-10 1.0gram 1grams 17.28 .07 340.8 213 154.91

10-3110-90 100µmoles .086grams 1.5 .07 25.2 15.75 11.45

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Related products

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