Cambio - Excellence in Molecular Biology

In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames MPF E.coli Combo Test Kits - semi-solid

The Ames MPF E.coli Combo Assay comprises the tryptophan auxotrophic tester strains E.coli wp2 [pKM101] and E.coli wp2 uvrA for detecting AT base-pair mutations at the primary reversion site as well as all media required to perform the assay

Xenometrix AG

Description

The Ames MPF E.coli Combo Assay comprises the tryptophan auxotrophic tester strains E.coli wp2 [pKM101] and E.coli wp2 uvrA for detecting AT base-pair mutations at the primary reversion site as well as all media required to perform the assay. Kits including additional S9 and Positive Controls are also available. E.coli wp2 strains detect certain oxidizing mutagens, cross-linking agents and hydrazines. The two strains have different sensitivities towards different mutagens. When combined in a given ratio for exposure, they detect a broader range of mutagens than the single strains alone .

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