Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

Tris-Glycine buffer pH 8.3

Tris-glycine buffer (TG) is the most common running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-12-9122-10Tris-Glycine buffer pH 8.3, 1000 ml 10 pouches £36.00 Quantity Add to Order
MC-12-9123-10Tris-Glycine buffer pH 8.3, 5000 ml 10 pouches £79.00 Quantity Add to Order

Tris-Glycine buffer pH 8.3

Tris-glycine buffer (TG) is the most common running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.

Medicago AB

Product description

Tris-glycine buffer (TG) is the most common running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.

TG buffer is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.

Medicago’s TG buffer are supplied as pre-weighed powder mixes in sealed pouches giving 1000 ml or 5000 ml of 0.025 M Tris, 0.192 M glycine with pH 8.3 at 25°C.

 

Features

  • Running buffers for protein electrophoresis
  • Formulated from analytical grade reagents
  • Assured consistency from lot to lot
  • Exactly pre-weighed powder mix in pouches
  • Dissolve and use in minutes

 

Directions for use

Empty one pouch of the TG or TG-SDS buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized
water and stir the solution for a few minutes. Adjust the volume up to 1000 ml or 5000 ml, stir until full dissolution and the buffer solution is ready to use.

 

Shipping and storage

The TG buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications                               

Chemicals: Analytical grade                   

Format: Exactly pre-weighed powder mix                  

Concentration: 0.025 M Tris, 0.192 M glycine                                            

Volume: 1000 ml and 5000 ml                  

pH: 8.3 ± 0.05 at 25°C       

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Tris-Glycine buffer pH 8.3

Tris-glycine buffer (TG) is the most common running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.

Medicago AB

Applications

  • Protein electrophoresis
  • Denatured protein electrophoresis
  • Polyacrylamide gel electrophoresis
  • Western blotting

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200