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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Vicia ervilia lectin (VEA)

Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for a-mannose and, to a lesser extent, a-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, a, a´-trehalose

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0114-10Vicia ervilia lectin (VEA) 10 mg £161.00 Quantity Add to Order
MC-05-0114-50Vicia ervilia lectin (VEA) 50 mg £574.00 Quantity Add to Order

Vicia ervilia lectin (VEA)

Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for a-mannose and, to a lesser extent, a-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, a, a´-trehalose

Medicago AB

Product description

Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for α-mannose and, to a lesser extent, α-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, α, α´-trehalose (1).

Medicago’s VEA lectin is supplied as a yellow lyophilized powder without preservatives. The purity of VGA is determined by SDS- electrophoresis. The lectin is available in vials containing 50 mg or 10 mg powder and the product is to be used for laboratory work only.

  

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

 

 

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Vicia ervilia lectin (VEA)

Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for a-mannose and, to a lesser extent, a-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, a, a´-trehalose

Medicago AB

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Vicia ervilia lectin (VEA)

Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for a-mannose and, to a lesser extent, a-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, a, a´-trehalose

Medicago AB

Specifications

Appearance: Yellow lyophilized powder or flocculate

Source: Bitter vetch seeds

Molecular weight: 53 kDa

Sugar specificity: α-Man > α-Glu

Activity: Non-specific after neuraminidase treatment of the blood cells.

Microorganisms: < 100 CFU/g

Protein content: > 90 %, OD280nm (ε 1.6 = 1 mg/mL)

Identity: SDS-PAGE

Shelf life: > Three years when stored at -20°

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Vicia ervilia lectin (VEA)

Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for a-mannose and, to a lesser extent, a-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, a, a´-trehalose

Medicago AB

Applications

  • Membrane protein studies

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200