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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-THP-Cyclo-dG-CE Phosphoramidite

5'-THP-Cyclo-dG-CE Phosphoramidite

Glen Research

Description

5'-THP-Cyclo-dG-CE Phosphoramidite

Structure

Catalog Number: 10-1598-xx

Description: 5'-THP-Cyclo-dG-CE Phosphoramidite

5'-O-Tetrahydropyran-2-yl-N-isobutyryl-(5'S)-8,5'-cyclo-
2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-
phosphoramidite
Formula: C28H42N7O7P M.W.: 619.65 F.W.: 327.19
Diluent: Anhydrous Acetonitrile
Coupling: 15 minute coupling time is recommended for Cyclo-dG; 6 minutes for base immediately following it. NOTE removal of the 5'-THP requires a 30 minute treatment with deblock. Please contact Technical Support for details.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

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Protocols

Material Safety Data Sheet

Glen Report 25.1: New Product - (5'S)-5',8-Cyclo-dG - DNA Damage and Repair


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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1598-90 100µmoles .062grams 1 20 12 7.5 5.45 4 1
10-1598-95 50µmoles .031grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1598-90 100µmoles .062grams 1.5 .07 23.6 14.75 10.73 1.48
10-1598-95 50µmoles .031grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1598-90 100µmoles .062grams 1.5 .07 25.2 15.75 11.45

10-1598-95 50µmoles .031grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200