Maximized packaging efficiency of lambda, cosmid, or fosmid DNA for the construction of large insert genomic libraries.
- Construct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.
- Packaging efficiencies of up to 3 × 109 pfu/µg DNA.
- Simplified protocol does not require premixing of different extract components before use.
stating actual packaging efficiency (pfu/µg DNA) for each respective lot of MaxPlax™ Extract is provided.
MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.
Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 × 109 pfu/µg of Control Ligated Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.
| Cosmid Insert |
Pfu/µg DNA |
| Unmethylated DNA |
3.5 x 107 |
| Methylated DNA |
3.9 x 107 |
References
- Hohn, E.G. (1979) Methods Enzymol. 68, 299.
- Gunther, E.G. et al. (1993) Nucleic Acids Res. 21, 3903.
- Fiandt, M. (2000) Epicentre Forum 7(3), 14
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