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Oligo Synthesis

Oligo Synthesis : CEPs

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COT Serinol Phosphoramidite

COT Serinol Phosphoramidite

Glen Research

Description

COT Serinol Phosphoramidite

3-Dimethoxytrityloxy-2-(3-(3-cyclooctatetraenylpropanamido)propanamido)propyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-
phosphoramidite

Formula: C47H59N4O7P 
M.W.: 822.97 
F.W.: 382.35
 
Please Note: This product is covered by US Patent Nos.: 8,394,948 and 8,945,515 B2.
 
COT Serinol Phosphoramidite
 
Bright, long-lasting and non-phototoxic organic fluorophores are essential for the continued optimization of a diverse range of imaging applications. However, all currently available technologies remain susceptible to undesirable transitions to dark states. Dark states arise from non-fluorescent triplet electronic configurations from which the rate of return to the ground state is slow. When in the triplet state, the fluorophore is susceptible to photobleaching and fluorescence applications are compromised by unpredictably reducing the signal-to-noise ratio (SNR), as well as limiting the total duration of time over which information can be gathered. The direct conjugation of small-molecule protective agents (PAs) has enabled significant improvements through intra-molecular triplet quenching. Through a partnership with Lumidyne Technologies, Glen Research has created a novel PA-linked phosphoramidite using cyclooctatetraene (COT). COT Serinol Phosphoramidite provides a means to improve the photostability of virtually any fluorophore in a modular fashion. Our spectrofluorometric studies show that the presence of COT limited the amount of photobleaching of an oligo containing the cyanine 5 dye.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

Glen Report 27.2: New Product - COT Serinol Phosphoramidite

 
Please Note: This product is covered by US Patent Nos.: 8,394,948 and 8,945,515 B2.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Diluent: Anhydrous Acetonitrile

Coupling: 3 minute coupling time recommended.

Deprotection:  No changes needed from standard method recommended by synthesizer manufacturer. NOTE: a side-reaction can occur post-synthesis that leads to cleavage of phosphodiester linkages vicinal to the COT. To avoid this side-reaction, deprotect the oligo soon after the oligonucleotide synthesis is completed.  Otherwise, store the column at -20°C until the deprotection can be initiated.  Avoid drying down the oligo with heat.

Storage: Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Please Note: This product is covered by US Patent Nos.: 8,394,948 and 8,945,515 B2.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200