The PseudoU-RNA Molecular Weight Markers are comprised of 7 bands of pseudouridine (ψ)-containing, single-stranded RNAs of the following sizes: 500, 1,000, 1,500, 2,000, 2,500, 3,000 and 4,000 nucleotides. RNA containing ψ (ψ-RNA) in place of the canonical uridine (U-RNA) displays altered mobility during electrophoresis as compared to the comparable U-RNA of identical sequence. The PseudoU-RNA Molecular Weight Markers allow users to more accurately estimate the size of ψ-containing RNAs.
Storage Buffer
The PseudoU-RNA Molecular Weight Markers are provided in 0.1 mM EDTA.
Functional Testing
The PseudoU-RNA Molecular Weight Markers are functionally tested by denaturing agarose gel electrophoresis analysis compared to a uridine-containing RNA molecular weight markers and discrete transcripts made with either ΨTP or UTP.
Contaminating Activity Assays
The PseudoU-RNA Molecular Weight Markers are free of detectable RNase activity.
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