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Oligo Synthesis

Oligo Synthesis : CEPs

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def-dA-CE Phosphoramidite

def-dA-CE Phosphoramidite 5'-Dimethoxytrityl-N-diethylformamidine-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1504-02def-dA-CE Phosphoramidite0.25g £14.00 Quantity Add to Order
10-1504-05def-dA-CE Phosphoramidite0.5g £27.00 Quantity Add to Order
10-1504-10def-dA-CE Phosphoramidite1.0g £55.00 Quantity Add to Order

Description

def-dA-CEP

Formula: C45H57N8O6P 

M.W.: 836.97

F.W.: 313.21

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection:  In 30% ammonium hydroxide, deprotect for 2 hours at 65 °C. In 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA), synthesize using acetyl-protected dC (10-1015-xx) and deprotect at 65 °C for 20 minutes.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry and in the dark

Stability in Solution: 1-2 Days

 

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Protocols

download SDS

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References

Glen Report 28.1

1. B.C. Froehler, and M.D. Matteucci, Nucleic Acids Res, 1983, 11, 8031-6.

2. L.J. McBride, R. Kierzek, S.L. Beaucage, and M.H. Caruthers, J. Amer. Chem. Soc., 1986, 108, 2040.

3. The Glen Report, 2009, 21.2, 14-15.

4. E.M. LeProust, et al., Nucl Acid Res, 2010, 38, 2522-2540.

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Notes

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack Size Grams/ Pack 0.1M Dil. (mL) LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1504-02 0.25grams .25grams 2.99 86.33 51.8 32.38 23.55 17.27 4.32
10-1504-02 0.25grams .25grams 2.99 86.33 51.8 32.38 23.55 17.27 4.32
10-1504-02 0.25grams .25grams 2.99 86.33 51.8 32.38 23.55 17.27 4.32
Expedite
Cat.No. Pack Size Grams/ Pack Dilution (mL) Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1504-02 0.25grams .25grams 4.46 .07 82.8 51.75 37.64 5.18  
10-1504-02 0.25grams .25grams 4.46 .07 82.8 51.75 37.64 5.18  
10-1504-02 0.25grams .25grams 4.46 .07 82.8 51.75 37.64 5.18  
Mermade
Cat.No. Pack Size Grams/ Pack Dilution (mL) Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1504-02 0.25grams .25grams 4.46 .07 84.4 52.75 38.36    
10-1504-02 0.25grams .25grams 4.46 .07 84.4 52.75 38.36    
10-1504-02 0.25grams .25grams 4.46 .07 84.4 52.75 38.36

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200