Cambio - Excellence in Molecular Biology

Protein Purification

Protein Purification: Glutathione Agarose

Glutathione Agarose (for GST-tagged protein purification)

Cambio Glutathione Agarose is a robust glutathione agarose resin that has high performance in purification of glutathione-S-transferase (GST)-tagged proteins.  It can be used for chromatography (column or batch) or in GST pull-downs (to validate or screen for interacting partners). Cambio Glutathione Agarose is made using high performance BioWorks Workbeads that are suitable for FPLC applications. Check out also our Glutathione Magnetic beads that we recommend for pull-down applications.

Glutathione Agarose

The GST tag is a 26 kDa protein (glutathione-S-transferase) that binds to glutathione-coupled agarose resins. This tag is considerably larger than the typically 6x or 10x His-tags and is often chosen to promote recombinant protein folding. Cambio Glutathione Agarose is produced using BioWorks Workbeads that have exceptional chromatography characteristics.,,,

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-224721Glutathione Agarose 10ml POA Quantity Add to Order
CA-224735Glutathione Agarose 50 ml POA Quantity Add to Order
CA-224770Glutathione Agarose 250ml POA Quantity Add to Order

Description

Glutathione Agarose

The GST tag is a 26 kDa protein (glutathione-S-transferase) that binds to glutathione-coupled agarose resins. This tag is considerably larger than the typically 6x or 10x His-tags and is often chosen to promote recombinant protein folding. Cambio Glutathione Agarose is produced using BioWorks Workbeads that have exceptional chromatography characteristics.

For purification from cell culture supernatants or pull-down experiments, we recommend Cambio Glutathione MagBeads.

Cambio Glutathione Agarose provides:

  • protein binding capacity of up to 10 mg/mL
  • compatible with a range of detergents and additives
  • ideal for purifying insoluble proteins

Cambio Glutathione Agarose has been shown to purify GST-tagged proteins with comparable yields and purity to leading brands available from other suppliers.

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