Cambio - Excellence in Molecular Biology

Transfection-Biontex

Transfection-Biontex: General transfection reagents

Broad range transfection reagents for a variety of mammalian and insect cells

Metafectene® Pro Transfection Reagent

Metafectene® PRO Transfection Reagent is the latest generation transfection reagent setting a new benchmark in mammalian cell transfection

Biontex

Catalogue No.DescriptionPack SizePriceQty
T040-1.0Metafectene™ Pro1ml £219.00 Quantity Add to Order
T040-2.0Metafectene™ Pro2 x 1ml £433.00 Quantity Add to Order
T040-5.0Metafectene™ Pro5 x 1ml £990.00 Quantity Add to Order

Description

 

Metafectene® PRO is the latest generation transfection reagent setting a new benchmark in mammalian cell transfection. 

Its novel composition combines Biontex' RMA-Technology ("Repulsive Membrane Acidolysis") with a new Toxicity Optimization module. (TOP-Technology) 

Decreasing the toxic effects is one of the leading aims in the transfection technology. In-vitro applications normally benefit from lower toxicity but in-vivo applications must. The development of our TOP-Technology furthermore fits another great advantage: it moderately destabilizes the DNA-lipid-complex and potentiates the depletive access for the RMA! 

Both techniques are excellently coordinated step-by-step. So this new product development consequently ensures the required release of genetic material in the cytoplasma and finally guarantees the availability of much more genetic material for uptake into the cell nucleus. 

Previous data support the fact that at least equivalent results can be achieved with METAFECTENE® PRO in comparison with Metafectene®, but multiple enhancement of efficiency can be obtained with moderate- or hard-to-transfect cell lines.

Metafectene® PRO is provided as a ready-to-use solution. It shows no serum inhibition, which makes it a reagent of choice for transfecting sensitive cell lines. 

Depending on the cell line, 1ml of Metafectene® PRO is sufficient for 50-200 transfections on 35 mm dishes (6-well plates) or 15 to 70 transfections on 60mm dishes.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Also available in size 0.2ml (T040-0.2) Please contact us for pricing

Product Specifications 
Application Transfection of mammalian cells with nucleic acids
Formulation Cationic lipids with colipids in water
Assays Approx. 500 (24-well) or approx. 200 (6-well) per 1 ml reagent
Shipping At room temperature
Storage ≤ –15°C
Shelf Life 1 year (after date of delivery)

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Application

  • Transfection of eukaryotic cells
  • Benefits

  • Improved toxicology (patented TOP Technology)
  • Enables very high expression rates per cell to be achieved
  • Comprehensive library of user reports
  • Extremely suitable for bacmid transfections (single-component system, no BacDNA precipitate formation

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200