Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
U-937 Cell Avalanche™ Transfection Reagent (human lymphoma cell)
ransfection of suspension cells, such as U-937 Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.
U-937 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on U-937 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U-937 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of U-937 Cell Avalanche™ Transfection Reagent for transfection on U-937 Cells.
EZ Biosystems
Description
| Catalog Number:EZT-U937-1 |
| Cell Synonyms:CRL-1593.2 , U937, U 937 |
Cell Line Information:
Designations: U-937
Morphology: monocyte
Source: histiocytic lymphoma
Culture Properties: suspension
Cellular Products:
lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis
factor (TNF) after stimulation with phorbol myristic acid (PMA)
Age: 37 years
Gender: male
Ethnicity: Caucasian
The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from
malignant cells obtained from the pleural effusion of a patient with
histiocytic lymphoma.
Studies since 1979 have shown that U-937 cells can be induced to
terminal monocytic differentiation by supernatants from human mixed
lymphocyte cultures,
phorbol esters, vitamin D3, gamma interferon, tumor necrosis factor
(TNF) and, retinoic acid. The cells express the Fas antigen, and are
sensitive to TNF and anti-Fas antibodies.
The Transfection Reagent:
Transfection
of suspension cells, such as U-937 Cells, has always been challenging,
both technically and financially. The most common systems currently used
for relatively efficient molecular delivery to these cells are
expensive electroporation instruments.
U-937
Cell Avalanche™ Transfection Reagent is a new, proprietary solution
specifically designed for transfection on U-937 Cells. The proprietary
formulation of lipids and polymers ensures the highest possible
transfection efficiencies and viabilities for U-937 Cells. No other
transfection reagents can match the efficiency, convenience, and
gentleness of U-937 Cell Avalanche™ Transfection Reagent for
transfection on U-937 Cells. For details of the developing process of
this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
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Specifically optimized to deliver nucleic acids into U-937 Cells
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Highest efficiency to ensure experimental success
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Extremely gentle to cells
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0.5 ml is able to transfect about 1000 wells of 24-well plate
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Animal-free
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Suitable for Reverse Transfection
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Compatible with transfection in any plate formats
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Economical: High efficiency means less amount of nucleic acid & reagent is needed
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Developed and manufactured by EZ Biosystems
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| Data: |
 |
| FIG. 1. High
throughput test of transfection efficiency (determined as RLU/mg) on
U-937 cells after transfection of luciferase reporter gene by using our
172 proprietary transfection formulas and several most popular
commercial transfection reagents. The yellow box showed the results of 4
commercial transfection reagents. The red lines marked our candidate
formulas with the highest transfection efficiency for U-937 cells. This
test result was confirmed with repeat experiments. The one that showed
the optimal balance of potent & low cytotoxicity among those
candidate formulas after flow cytometry analysis on the percentage of
7AAD positive cells was later named as this U-937 Cell Avalanche
Transfection Reagent. |
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For Other Cells: |
U-937 Cell
Avalanche™ Transfection Reagent (human lymphoma cell) can also be used
on the following cells with high transfection efficiencies.
293 Cell
293T/17 Cell
NIH/3T3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
CT26.WT Cell
NCTC Clone 929 Cell
Recommended protocols for these cells will be provided with the
reagent. The protocols usually provide satisfactory transfection
efficiency with invisible cytotoxicity. However, optimization may be
needed for certain type of cells. Optimizations may include: the amount
of DNA and this transfection reagent; cell density; transfection
reagent/DNA ratio, or incubation time for the mixture of transfection
reagent/DNA etc. For best transfection result, we recommend using the
respective cell type/cell line specific Avalanche transfection reagents.
Those reagents have been optimized on both recipes and protocols, and
have been proved to have the best transfection results for the
respective cell lines or primary cells. You can easily find the
respective Avalanche transfection reagents specific for your cells by
using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200