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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-OMe-Ac-C-CE Phosphoramidite

2'-OMe-Ac-C-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3115-022'-OMe-Ac-C-CE Phosphoramidite 0.25g £23.00 Quantity Add to Order
10-3115-052'-OMe-Ac-C-CE Phosphoramidite 0.5g £45.00 Quantity Add to Order
10-3115-102'-OMe-Ac-C-CE Phosphoramidite 1.0g £91.00 Quantity Add to Order
10-3115-902'-OMe-Ac-C-CE Phosphoramidite 100µmoles £14.00 Quantity Add to Order

Description

2'-OMe-Ac-C-CE Phosphoramidite

Structure

Catalog Number: 10-3115-xx

Description: 2'-OMe-Ac-C-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-methyl,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C42H52N5O9P M.W.: 801.88 F.W.: 319.21
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 days

2’-Ome-rna phosphoramidites

Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

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Protocols

MSDS

Glen Report 8.1

Glen Report 15.1

Glen Report 19.2

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3115-02 0.25grams .25grams 3.12 90.67 54.4 34 24.73 18.13 4.53
10-3115-05 0.5grams .5grams 6.24 194.67 116.8 73 53.09 38.93 9.73
10-3115-10 1.0gram 1grams 12.47 402.33 241.4 150.88 109.73 80.47 20.12
10-3115-90 100µmoles .08grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3115-02 0.25grams .25grams 4.65 .07 86.6 54.13 39.36 5.41
10-3115-05 0.5grams .5grams 9.31 .07 179.8 112.38 81.73 11.24
10-3115-10 1.0gram 1grams 18.61 .07 365.8 228.63 166.27 22.86
10-3115-90 100µmoles .08grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3115-02 0.25grams .25grams 4.65 .07 88.2 55.13 40.09

10-3115-05 0.5grams .5grams 9.31 .07 181.4 113.38 82.45

10-3115-10 1.0gram 1grams 18.61 .07 367.4 229.63 167

10-3115-90 100µmoles .08grams 1.5 .07 25.2 15.75 11.45

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Related products

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