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Oligo Synthesis

Oligo Synthesis : CEPs

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5-I-dC-CE Phosphoramidite

5-I-dC-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1081-025-I-dC-CE Phosphoramidite 0.25g £284.00£284.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
10-1081-905-I-dC-CE Phosphoramidite 100µmoles £108.00£108.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order

5-I-dC-CE Phosphoramidite

5-I-dC-CE Phosphoramidite

Glen Research

5-I-dC-CE Phosphoramidite

Catalog Number: 10-1081-xx

Description: 5-I-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-5-iodo-2'-deoxyCytidine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C46H51IN5O8P M.W.: 959.83 F.W.: 415.08

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Mild deprotection: Ammonium Hyrdoxide for 24 hours at room temperature.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours

HALOGENATED NUCLEOSIDES

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

Structure

HALOGENATED NUCLEOSIDES

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

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5-I-dC-CE Phosphoramidite

5-I-dC-CE Phosphoramidite

Glen Research

MSDS

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5-I-dC-CE Phosphoramidite

5-I-dC-CE Phosphoramidite

Glen Research

Oligonucleotides containing a bromo or iodo group are prepared conventionally with the exception that deprotection is carried out in ammonium hydroxide at room temperature for 24 hours.  Under these conditions, degradation of the halogen group was less than 2%.

Frequently Asked Technical Question

QUESTION: What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?

RESPONSE:See: Table of Extinction Coefficients

REFERENCE(S):
B. Froehler, Gilead
M. Powell
R. Somers

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5-I-dC-CE Phosphoramidite

5-I-dC-CE Phosphoramidite

Glen Research

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1081 5-I-dC 293 5.7

3.3

 

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1081-02 0.25grams .25grams 2.6 73.33 44 27.5 20 14.67 3.67
10-1081-90 100µmoles .096grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1081-02 0.25grams .25grams 3.89 .07 71.4 44.63 32.45 4.46
10-1081-90 100µmoles .096grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1081-02 0.25grams .25grams 3.89 .07 73 45.63 33.18

10-1081-90 100µmoles .096grams 1.5 .07 25.2 15.75 11.45

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