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Oligo Synthesis

Oligo Synthesis : CEPs

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5-I-U-CE Phosphoramidite

5-I-U-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3091-025-I-U-CE Phosphoramidite 0.25g £536.00 Quantity Add to Order
10-3091-905-I-U-CE Phosphoramidite 100µmoles £218.00 Quantity Add to Order
10-3091-955-I-U-CE Phosphoramidite 50µmoles £123.00 Quantity Add to Order

Description

5-I-U-CE Phosphoramidite

Structure

Catalog Number: 10-3091-xx

Description: 5-I-U-CE Phosphoramidite

5'-Dimethoxytrityl-5-iodo-2'-Uridine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C45H60N4O9PISi M.W.: 986.96 F.W.: 432.07
Diluent: Anhydrous Acetonitrile
Coupling: 12 minute coupling time
Deprotection: Ammonium hydroxide/Ethanol 3:1 (v/v) at room temperature as required by the nucleobases. Complete the 2' deprotection using standard RNA protocols. See www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf. Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

minor rna phosphoramidites (TBDMS PROTECTED)

Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships. 5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments. 6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used. 2-Aminopurine riboside is useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3091-02 0.25grams .25grams 2.53 71 42.6 26.63 19.36 14.2 3.55
10-3091-90 100µmoles .099grams 1 20 12 7.5 5.45 4 1
10-3091-95 50µmoles .049grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3091-02 0.25grams .25grams 3.78 .07 N/A N/A 14.42 N/A
10-3091-90 100µmoles .099grams 1.5 .07 N/A N/A 4.92 N/A
10-3091-95 50µmoles .049grams .75 .07 N/A N/A 1.79 N/A
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3091-02 0.25grams .25grams 3.78 .07 N/A 11.42 10.73

10-3091-90 100µmoles .099grams 1.5 .07 N/A 4.06 3.82

10-3091-95 50µmoles .049grams .75 .07 N/A 1.65 1.55

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Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200