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Oligo Synthesis

Oligo Synthesis : CEPs

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Ac-dC-CE Phosphoramidite

Ac-dC-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1015-02Ac-dC-CE Phosphoramidite 0.25g £10.00£8.50Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-02MAc-dC-CE Phosphoramidite 0.25g £10.00£8.50Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-05Ac-dC-CE Phosphoramidite 0.5g £20.00£17.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-05MAc-dC-CE Phosphoramidite 0.5g £20.00£17.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-10Ac-dC-CE Phosphoramidite 1.0g £40.00£34.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-10MAc-dC-CE Phosphoramidite 1.0g £40.00£34.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-1CAc-dC-CE Phosphoramidite 1.0g £40.00£34.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-1SAc-dC-CE Phosphoramidite 10.0g £400.00£340.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-20Ac-dC-CE Phosphoramidite 2.0g £80.00£68.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-2CAc-dC-CE Phosphoramidite 2.0g £80.00£68.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-40Ac-dC-CE Phosphoramidite 4.0g £160.00£136.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-50Ac-dC-CE Phosphoramidite 5.0g £200.00£170.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-5SAc-dC-CE Phosphoramidite 5.0g £200.00£170.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-C2Ac-dC-CE Phosphoramidite 0.25g £10.00£8.50Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order
10-1015-C5Ac-dC-CE Phosphoramidite 0.5g £20.00£17.00Offer until : 30-Sep-2019Offer Code : GLEN1515% offer orders over £1000 View Offer Quantity Add to Order

Ac-dC-CE Phosphoramidite

Ac-dC-CE Phosphoramidite

Glen Research

Ac-dC-CE Phosphoramidite

Structure

Catalog Number: 10-1015-xx

Description: Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C41H50N5O8P M.W.: 771.85 F.W.: 289.18

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ac-dC-CE Phosphoramidite

Ac-dC-CE Phosphoramidite

Glen Research

Frequently Asked Technical Question

QUESTION: How does this work? What would happen if I used the AMA reagent with Bz protected dC?

RESPONSE:dC when proected as an amide linkage is susceptible to transamination or displacement of the amide with the deprotecting amine. Normally, the deprotecting amine is ammonia and dispacement leads to the same product as hydrolysis. However, if methylamine is used for the deprotection step with a benzoyl protected dC, approximately 10% of the product is from the displacement reaction to give N-methyl-dC residues, while the remaining 90% is the desired product formed by hydrolysis. A more labile protecting group on dC is isobutyryl which is also commercially available. This group is displaced by methylamine to about 5% - an improvement but still a mutation. Ac-dC, on the other hand, is hydrolyzed virtually instantaneously by the AMA reagent and none of the slower displacement reaction gets a chance to occur.

REFERENCE(S):
M.P. Reddy, Beckman Instruments, personal communication.


QUESTION: Can the AMA reagent be made up and stored? for how long? in what kind of container?

RESPONSE:Yes. Store tightly sealed in a glass bottle in the refrigerator. The reagent does not decompose but routine opening will lose some of the volatile bases. Dispose properly after 4-6 weeks.

REFERENCE(S):
Candy Lee, Dartmouth


QUESTION: What is the procedure for deprotecting with AMA?

RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium

hydroxide and aqueous MethylAmine. With AMA the cleavage of the

oligonucleotide from the support is accomplished in 5 minutes at room

temperature. The deprotection step is carried out at 65°C for a

further 5 minutes. Deprotection can also be carried out at lower

temperatures as shown in Table 1. In all cases, no base modification

has been observed.

TABLE 1: DEPROTECTION WITH AMA

 

Time Temperature
5 min 65°C
10 min 55°C
30 min 37°C
90 min 25°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ac-dC-CE Phosphoramidite

Ac-dC-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1015-02 0.25grams .25grams 3.24 94.67 56.8 35.5 25.82 18.93 4.73
10-1015-05 0.5grams .5grams 6.48 202.67 121.6 76 55.27 40.53 10.13
10-1015-10 1.0gram 1grams 12.96 418.67 251.2 157 114.18 83.73 20.93
10-1015-20 2.0grams 2grams 25.91 850.33 510.2 318.88 231.91 170.07 42.52
10-1015-40 4.0grams 4grams 51.82 1714 1028.4 642.75 467.45 342.8 85.7
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1015-1C 1.0gram 1grams 19.34 .07 383.6 239.75 174.36 23.98  
10-1015-2C 2.0grams 2grams 38.67 .07 770.2 481.38 350.09 48.14  
10-1015-C2 0.25grams .25grams 4.83 .07 93.4 58.38 42.45 5.84  
10-1015-C5 0.5grams .5grams 9.67 .07 190.2 118.88 86.45 11.89  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1015-1B 1.0gram 1grams 19.34 .07 382 238.75 173.64    
10-1015-B2 0.25grams .25grams 4.83 .07 91.8 57.38 41.73    
10-1015-B5 0.5grams .5grams 9.67 .07 188.6 117.88 85.73

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200