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Oligo Synthesis

Oligo Synthesis : Supports, CPGs

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dI-CPG 500

dI-CPG 500

Glen Research

Description

dI-CPG 500

Structure

 

Catalog Number: 20-2040-xx

(20-2190-xx)

Description: dI-CPG 500

5'-Dimethoxytrityl-2'-deoxyInosine,3'-succinoyl-long chain alkylamino-CPG 500
    F.W.: 314.19

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Not Applicable

duplex effects

The design of primers is frequently complicated by the degeneracy of the genetic code. Three strategies are now available to confront this problem. In the first, a mixed base addition (N) is used to form the degenerate site. This approach is best if the number of degenerate sites is small. A second option is the use of 2’-deoxyInosine or 2’-deoxyNebularine which exhibit low, but unequal, hydrogen bonding to the other four bases. The third option is the use of a universal nucleoside. In this strategy, the base analog does not hybridize significantly to the other four bases and makes up some of the duplex destabilization by acting as an intercalating agent. 3-Nitropyrrole 2’-deoxynucleoside (M) is the first example of a set of universal bases. Subsequently, 5-nitroindole was determined to be an effective universal base and to be superior to 3-nitropyrrole, based on duplex melting experiments.

The modified bases designated P and K show considerable promise as degenerate bases. The pyrimidine derivative P, when introduced into oligonucleotides, base pairs with either A or G, while the purine derivative K base pairs with either C or T. A dP dK mix also can serve as a mixed base with much less degeneracy than dA dC dG dT (N).

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Protocols

Material Safety Data Sheet

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Applications & Benefits

The design of primers is frequently complicated by the degeneracy of the genetic code.  Three strategies are now available to confront this problem. 

  1. A mixed base addition (N) is used to form the degenerate site.  This approach is best if the number of degenerate sites is small.
  2. The use of 2'-deoxyInosine or 2'-deoxyNebularine which exhibit low, but unequal, hydrogen bonding to the other 4 bases.
  3. The use of a universal nucleotide.  In this strategy, the base analog does not hybridise significantly to the other 4 bases and makes up some of the duplex destabilisation by acting as an intercalating agent.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200