Cambio - Excellence in Molecular Biology

In vitro Transcription

In vitro Transcription: In Vitro Transcription

PseudoU-RNA Molecular Weight Markers

The PseudoU-RNA Molecular Weight Markers are comprised of 7 bands of pseudouridine (?)-containing, single-stranded RNAs, of the following sizes: 500, 1,000, 1,500, 2,000, 2,500, 3,000 and 4,000 nucleotides.

CellScript

Description

The PseudoU-RNA Molecular Weight Markers are comprised of 7 bands of pseudouridine (ψ)-containing, single-stranded RNAs of the following sizes: 500, 1,000, 1,500, 2,000, 2,500, 3,000 and 4,000 nucleotides. RNA containing ψ (ψ-RNA) in place of the canonical uridine (U-RNA) displays altered mobility during electrophoresis as compared to the comparable U-RNA of identical sequence. The PseudoU-RNA Molecular Weight Markers allow users to more accurately estimate the size of ψ-containing RNAs.

Storage Buffer
The PseudoU-RNA Molecular Weight Markers are provided in 0.1 mM EDTA.


Functional Testing
The PseudoU-RNA Molecular Weight Markers are functionally tested by denaturing agarose gel electrophoresis analysis compared to a uridine-containing RNA molecular weight markers and discrete transcripts made with either ΨTP or UTP.


Contaminating Activity Assays
The PseudoU-RNA Molecular Weight Markers are free of detectable RNase activity.

 

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Protocols

Download protocol here

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Notes

Store at –20oC or –70oC in a freezer without a defrost cycle.

Use 1 μl per lane. 25 lanes per package.
1 μl of the PseudoU-RNA Molecular Weight Markers contains 50 ng/μl of each band.

Materials Required, but not Supplied:

• RNA gel loading buffer
• RNase-free, Water or TE Buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA)

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200