Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Plasmid

Mini, midi and maxi preps including rapid plasmid prep kits.

Exfection™ Plasmid LE

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase

GeneAll

Catalogue No.DescriptionPack SizePriceQty
GA-111-102GeneAll Exfection Plasmid LE 200 mini preps £164.00 Quantity Add to Order
GA-111-110GeneAll Exfection Plasmid LE 10 prep sample (1 per customer) £0.00 Quantity Add to Order
GA-111-150GeneAll Exfection Plasmid LE 50 mini preps £75.00 Quantity Add to Order
GA-111-201GeneAll Exfection Plasmid LE 100 midi prep £640.00 Quantity Add to Order
GA-111-226GeneAll Exfection Plasmid LE 26 midi preps £204.00 Quantity Add to Order
GA-111-701Spin Column for Exfection LE/EF Midi25 each £80.00 Quantity Add to Order
GA-111-901Buffer P1 60 ml £19.60 Quantity Add to Order
GA-111-903Buffer P1 100 ml £22.00 Quantity Add to Order
GA-111-911Buffer P2 60 ml £20.80 Quantity Add to Order
GA-111-913Buffer P2 100 ml £23.20 Quantity Add to Order
GA-111-921Buffer G3 90 ml £32.80 Quantity Add to Order
GA-111-922Buffer G3 110 ml £38.80 Quantity Add to Order
GA-111-933Buffer EW1 150ml £40.00 Quantity Add to Order
GA-111-935Buffer EW1 300 ml £63.00 Quantity Add to Order
GA-111-944Buffer EW2 250 ml £8.00 Quantity Add to Order
GA-111-946Buffer EW2 400 ml £13.00 Quantity Add to Order
GA-111-952Buffer EF 60 ml £17.20 Quantity Add to Order
GA-111-991Spin Column for Exfection LE mini100 each £40.00 Quantity Add to Order
GA-111-992Spin Column for Exfection LE mini200 each £66.00 Quantity Add to Order

Exfection™ Plasmid LE

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase

GeneAll

Description

ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the
purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/ug with ExfectionTM EF and to 10 EU/ug with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.

 

Features and Benefits

¦ Spin column format based on glassfiber membrane
¦ Convenient clearing of lysate with EzClearTM filter (Midi)
¦ High plasmid recoveries with high purity
¦ Fast preparation time and simple procedure
¦ High transfection efficiency in most cell-lines
¦ No need of additional materials
¦ No use of organic solvents

 

Procedure


 

Specification

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exfection™ Plasmid LE

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase

GeneAll

Click here fro protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exfection™ Plasmid LE

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase

GeneAll

List of references here

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200