QuickExtract™ Plant DNA Extraction Solution
Rapid and efficient extraction of PCR-ready genomic DNA from plant leaf samples
- Fast: PCR-ready DNA in about 8 minutes for most samples
- Simple: No bead-beating, freezing, or grinding of plant leaf material
- Increased Yields: No centrifugation or spin columns used which reduce yields
- Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
- Safe: Nontoxic reagets used throughout the procedure
he QuickExtract™ Plant DNA Extraction Solution can be used to rapidly
and efficiently extract PCR-ready genomic DNA from most plant leaf
samples using a simple, one-tube protocol that takes only 8 minutes
(Fig. 1). Most leafy plants are suitable for DNA extraction using the
QuickExtract Plant Solution, including Arabidopsis, barley, maize,
emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).
The
QuickExtract Plant method allows for the inexpensive processing of one
to hundreds of samples simultaneously, without grinding the sample,
centrifugation, spin columns, or any toxic organic solvent. The
procedure is fully compatible with robotic automation, provides a
PCR-ready sample, and is reproducible (Fig. 3). Simply add the
QuickExtract Plant solution to the sample and perform two sequential
heating steps. A small aliquot of the sample is then used as a template
for PCR or qPCR.
Applications
- High-throughput isolation of DNA from plant leaf samples for PCR-based analysis, e.g., GMO testing.
| 
Figure 1 (click to enlarge). Overview of the QuickExtract™ Plant DNA extraction procedure. |
| 
| Figure 2. PCR products using QuickExtract™ Plant DNA Extraction Solution with different varieties of plant leaves.
Forty cycles of RAPD were performed with DNA extracted from plant
leaves using the QuickExtract Plant solution and the FailSafe™ PCR
System. Lane M, 100-bp ladder; lane 1, pepper; lane 2, soybean; lane 3,
spelt. |
 | Figure 3. Reproducibility of PCR results with QuickExtract™ Plant DNA Extraction Solution from Arabidopsis thaliana leaves. Six individual punches of four Arabidopsis
leaves were treated with QuickExtract Plant solution as described in
the product information sheet. One microliter of the solution was used
in a 25-µL PCR using the FailSafe™ PCR System and primers specific for
the single-copy HSC70 chromosomal gene. Aliquots were analyzed on a 2%
agarose gel and the DNA was visualized by staining with SYBR® Gold.
Lanes 1-6, PCR products of extracted DNA samples; |
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