Cambio - Excellence in Molecular Biology

In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Micronucleus Test MNvit for detection of Aneugens and Clastogens

Fast Detection of True Positive Compounds. 1 hour analysis time with improved precision
Fastest FISH assay EVER!

Xenometrix AG

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • £
XM020Rapid MicroNucleus MoA Kit20 samples /kit POA Quantity Add to Order
XM100Rapid MicroNucleus MoA Kit100 samples /kit POA Quantity Add to Order

Description

Rapid MicroNucleus MoA –
Fast Detection of True Positive Compounds

Fastest FISH assay ever! - Fast, clear identification of Aneugens and Clastogens

 

Introducing the new  in vitro Human cell micronucleus test ( MNvit), that gives you results in 1 hour.  MNvit  is one of the most widely used in vitro genotoxcity assays combined with, or following the AMES test.

This is a method that provides a comprehensive basis for investigating chromosome damaging potential of a chemical or a radiation.  The Patent pending  MicroNucleus MoA kit provides additional information on the mechanisms of chromosome damage and micronucleus formation: aneugens and clastogens. 

It is also able to provide information on Chromosomal aberrationsand Identify agents that cause structural aberrations in cultured mammalian cells. The kit uses a very simple protocol with minimal additional requirements  and equipment already present in many laboratory environments.

Key features

  • 1 hour FISH Assay – fast, reliable genotoxicity assessment
  • High specificity – no false positive results due to RNA, low-density micronuclei, precipitates, cytoplasmic alterations
  • Ready to use kit including pan-centromeric probes, DAPI and reagents
  • Simultaneous DAPI Counterstain
  • Fast, clear identification of mode of action: Aneugens or Clastogens
  • FISH staining of centromeres is recommended in OECD TG487
  • Can be used for Chemicals, Pharmaceuticals, Cosmetics, environmental samples
  • For all human cells and whole blood

 

 

 

Overview of Procedure

Rapid Micronucleus MoA is a ready to use kit for the fast identification of aneugens and clastogens according to OECD 487. Cells are prepared as per standard protocols according to OECD TG 487 and the slides are sequentially treated with a series of solutions provided with the kit “Rapid Micronucleus MoA”, including a human-specific, highly sensitive centromere probe and a counterstaining solution containing DAPI (4’,6-diamidino-2-phenylindole). Slides are sequentially washed with PBS and an ethanol series after treatment. Centromere probes are then added to the area of interest and the slide is heated at 80°C for 3 minutes and transferred to a humidified chamber for 20 minutes. Washes and a 5-minutes counterstaining step follow prior to the addition of the mounting solution.


Using a 40× magnification objective it will be possible to easily identify micronuclei and assess whether a centromere is present. Automated reading and archiving can be obtained with the slide scanning systems, e.g. Metafer.
Slides are usually ready for evaluation within 1 hour. The application of FISH probes allows directly to distinguish micronuclei originating either from chromosome loss (aneugenic compounds) or breakage (clastogenic compounds).

FISH probe technology as used in the Rapid Micronucleus MoA Kit can also be used in chromosomal aberration tests (OECD 473), cytogenetic analysis in Research or in physical dosimetry, i.e. people exposed in medical or natural radiation.

The frequency of micronuclei has been used for many years as a biomarker to measure chromosomal damage according to guideline OECD TG487.

References

  • Cuceu C, Colicchio B, Jeandidier E, Junker S, Plassa F, Shim G, Mika J, Frenzel M, Al Jawhari M., Hempel WM, O'Brien G, Lenain A, Morat L, Girinsky T, Dieterlen A, Polanska J, Badie C, Carde P, M'Kacher R. 2018. Independent mechanisms lead to genomic instability in Hodgkin lymphoma: Microsatellite or Chromosomal. Cancers (Basel). 10(7).pii:E233. Erratum in: 2019. Cancers (Basel). 11(6).pii:E757.
  • Zaguia N., Laplagne E., Colicchio B., Cariou O., Al Jawhari M., Heidingsfelder L., Hempel WM, Bel Hadj Jrad B, Jeandidier E, Dieterlen A, Carde P, Voisin P, M’Kacher R. 2020. A new tool for genotoxic risk assessment: Reevaluation of the cytokinesis block micronucleus assay using semi-automated scoring following telomere and centromere staining. Mutat Res Gen Tox En. 850-851:503143.

 


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