Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Heat labile & Salt Tolerant Enzymes

New range of enzymes for 2019. Our new range of enzymes include novel enzymes specifically designed or engineered to be amazingly tolerant to high salt concentrations, function at very low temperatures and heat inactivated.

Cryophile Uracil DNA Glycosylase (heat-labile)

Cryophile Uracil DNA Glycosylase hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA. Cryophile Uracil DNA Glycosylase is not active on uracil in RNA. The high activity combined with a fast irreversibly heat-inactivation step (the enzyme is heat-labile) makes the enzyme ideal for contamination control in PCR and RT-PCR reactions. Unlike all other available UNG enzymes, Cryophile Uracil DNA Glycosylase does not reactivate after heat-inactivation. This enables contamination control in amplification reactions also where subsequent post-PCR analysis is required.

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-1727-100Cryophile Uracil DNA Glycosylase (heat-labile)100 U (1U/µl) £80.00 Quantity Add to Order
CA-1727-1000Cryophile Uracil DNA Glycosylase (heat-labile)1000U (1U/µl) £330.00 Quantity Add to Order
CA-1727-100TCryophile Uracil DNA Glycosylase (heat-labile)100 U(1U/µl) Triton Free £85.00 Quantity Add to Order

Cryophile Uracil DNA Glycosylase (heat-labile)

Cryophile Uracil DNA Glycosylase hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA. Cryophile Uracil DNA Glycosylase is not active on uracil in RNA. The high activity combined with a fast irreversibly heat-inactivation step (the enzyme is heat-labile) makes the enzyme ideal for contamination control in PCR and RT-PCR reactions. Unlike all other available UNG enzymes, Cryophile Uracil DNA Glycosylase does not reactivate after heat-inactivation. This enables contamination control in amplification reactions also where subsequent post-PCR analysis is required.

Cambio

Cryophile Uracil DNA Glycosylase

Cryophile Uracil DNA Glycosylase or Uracil-N glycosylase (UNG) hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil- containing DNA leaving a basic (apyrimidinic) site in DNA. Cryophile Uracil DNA Glycosylase is not active on uracil in RNA. The high activity combined with a fast irreversible heat-inactivation step makes the enzyme ideal for contamination control in PCR and RT-PCR reactions. Unlike all other available UNG enzymes, Cryophile Uracil DNA Glycosylase does not reactivate after heat-inactivation. This enables contamination control in amplification reactions also where subsequent post-PCR analysis is required.

Features

  • The only UNG that can be completely and irreversibly heat inactivated
  • Heat-labile without any addition of agents or inhibitors
  • Eliminates carry-over contamination in both PCR and RT-PCR
  • Enables downstream post-PCR analysis such as cloning and sequencing

Source

Arctic cod origin, recombinantly produced in E. coli.

Heat inactivation

Cryophile Uracil DNA Glycosylase is completely and irreversibly inactivated by incubating the enzyme at 55°C for 20 min.

Storage

Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.

Purity

Cryophile Uracil DNA Glycosylase highly pure and is tested free of contaminating nucleases.

Specific activity

> 500 000 Units/mg

Unit defnition

One unit of UNG is defined as the amount of enzyme required to release 1 nmol uracil from uracil-containing DNA per hour at 37°C

Use of Cryophile Uracil DNA Glycosylase in RT-PCR

A prerequisite for using UNG in RT-PCR contamination control, is that the enzyme is sufficiently heat-labile to quickly inactivate at the temperatures used for reverse transcription. The easily heat-inactivatable Cryophile Uracil DNA Glycosylase makes it possible to use contamination control also in RT-PCR, being able to remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay. Applying Cryophile Uracil DNA Glycosylase in an RT-PCR reaction is done by a 5 min Cryophile Uracil DNA Glycosylase preincubation step at room temperature prior to the amplification reaction. Cryophile Uracil DNA Glycosylase does not affect sensitivity of qRT-PCR.

PCR product stability after Cryophile Uracil DNA Glycosylase treatment

The complete and irreversible heat-inactivation of Cryophile Uracil DNA Glycosylase enables the combination of contamination control and an array of post-PCR applications such as cloning, sequencing or genotyping. As Cryophile Uracil DNA Glycosylase is the only commercially available UNG that does not reactivate post-amplification, PCR products remain intact and can be stored for a prolonged period of time before further analysis.

 

 

 

 

 

 

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