CviJ I is the only available restriction enzyme recognising two/three basepair sequence, thus allowing for new research applications.
This version of CviJ I cleaves only PuGCPy sites. It does not exhibit ‘star’ activity, thus it is better suited for mapping of short DNAs like PCR products or small plasmids. Other CviJ I applications, like shotgun cloning, thermal cycle labelling or epitope mapping, can be performed either with this version or with CviJ I*, also available from Cambio (Call for details).
Source
Chlorella Virus IL-3A
Unit definition
One unit is defined as the amount of enzyme required to completely digest 1µg of pBR322 DNA in 1 hour in a total reaction volume of 25µl.
| Assay Conditions |
20mM Glycylglycine-KOH (pH 8.5) |
| 0.1mM DTT |
| 50mM K-acetate |
| 10% DMSO |
| 10mM Mg-acetate |
| 1µg pBR322 DNA |
| Reaction volume 25µl |
| DMSO is supplied separately. |
| Storage Buffer |
20mM Tris-acetate (pH 8.0) |
| 50mM potassium acetate |
| 0.5mM EDTA |
| 1mM DTT |
| 5mM MgCl2 |
| 50% (v/v) glycerol |
Store enzyme at -80°C, buffer at -20°C and DMSO at Room Temp
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