Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent
Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Astrocytes. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Astrocytes.
EZ Biosystems
Description
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Human Astrocytes are the most abundant cells in the central nervous system. They perform many functions such as provision of mechanical support and nutrients to neurons, and removal of wastes from neurons. In addition to playing a critical support role for optimal neuronal functioning, they also provide biochemical support of endothelial cells which form the blood-brain barrier. Recent studies have shown that astrocytes are capable of regulating neurogenesis by instructing the stem cells to adopt a neuronal fate. They also respond to neurotransmitters and modulate neuronal excitability and synaptic function.
Human Astrocytes are usually derived from human cerebral cortex. They can serve as useful in vitro model for exploring the diversity of astrocytes functions.
Applications:
- Astrocyte-mediated neurotoxicity
- Neurogenesis research
- Injury
- Drug development
- Parkinson's disease
- Alzheimer's disease
The Transfection Reagent:
Transfection of primary cells, such as Human Astrocytes, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Astrocytes. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Astrocytes. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in Human Astrocytes.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human astrocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human astrocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Astrocyte Avalanche™ Transfection Reagent. |
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For Other Cells: |
Human Astrocyte (HA, NHA) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200