Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
10X Reaction Buffer: 0.5 M MOPS (pH 7.5), 0.1 M KCl, 50 mM MgCl2, and 10 mM DTT. The Reaction Buffer does not contain
ATP or MnCl2 which must be added to the reaction at final concentrations of 0.05 mM ATP and 2.5 mM MnCl2. A 2.5-mM solution of ATP and a 50-mM solution of MnCl2 are included.
Quality Control: CircLigase ssDNA Ligase is free of detectable phosphatase, DNA exo- and endonuclease, and RNase activities
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