Description
ExgeneTM Tissue SV kit provides a simple and rapid method for the isolation of total DNA from animal tissues and cultured cells. This kit can process 25 mg (mini) of wet tissue and yields up to 50 ug (mini) depending on the type of sample used. Specially formulated buffer system minimize RNA copurified with DNA without RNase A treatment. If RNA-free genomic DNA is required, RNase A can be treated with. No organic extraction and alcohol precipitation are needed and multiple samples can be easily processed simultaneously. ExgeneTM Tissue SV plus! offers additional material and method for DNA purification from whole blood.
Click here here for protocol
Features and Benefits
¦ Spin or vacuum column format
¦ Accurate and consistent DNA extraction from animal tissues, cultured cell line and whole blood (plus! only)
¦ Instant use : No need of additional materials
¦ Simple and safe procedure
¦ High purity : 1.8 ~ 2.0
¦ No use of organic solvents
¦ Ready for use in PCR, southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions
Technical Information
| Features |
Tissue SV mini |
Tissue SV Midi |
Tissue SV MAXI |
| Format |
Column type G (mini) with collection tube | Column type G (Midi) with collection tube | Column type G (MAXI) with collection tube |
| Maximum sample size | ~25 mg | ~100 mg | ~250 mg |
| Preparation time | 25~220 minutes | 40~250 minutes | 40~250 minutes |
| Maximum loading volume | 750 μl | 5 ml | 16 ml |
| Binding capacity | 60 μg | 200 μg | 600 μg |
| Elution volume | 30~400 μl | 200~600 μl | 0.4~2 ml |
Technical Data
Compatibility Test with Restriction Enzymes
PCR Amplification Test
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200