Description
Exgene™ Plant SV kit provides a simple and easy method for small, medium and large scale purification of total DNA from various plant tissues. With EzSep™ filter and column type G, the procedure can be completed in as little as 40 minutes for the mini-prep version, yielding genomic DNA of high purity which is suitable for use directly in most common downstream appilcations. Up to 100 mg, 400 mg of plant tissue can be processed with Exgene™ Plant SV mini, Midi and MAXI, respectively.
Features and Benefits
¦ Spin or vacuum column format
¦ Stable and consistent DNA extraction from plant cells, tissues and fungi
¦ Instant use : No need of additional materials
¦ Perfect removal of second metabolites such as polyphenols and polysaccharides
¦ Simple procedure by the use of EzSepTM filter column
¦ No use of organic solvents
¦ Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions
Protocol
| Features | Plant SV mini | Plant SV Midi | Plant SV MAXI |
|---|
| Format |
Column type G (mini) with collection tube | Column type G (Midi) with collection tube | Column type G (MAXI) with collection tube |
| Maximum sample size | | ~400 mg wet
(100 mg dry)
| ~1 g wet (250 mg dry) |
| Preparation time | <40 minutes | <60 minutes | <60 minutes |
| Maximum loading volume | 750 μl | 5 ml | 15 ml |
| Typical yield | 4~40 μg | 10~150 μg | 40~300 μg |
| Elution volume | 30~400 μl | 200~600 μl | 0.4~2 ml |
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