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Oligo Synthesis

Oligo Synthesis : CEPs

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5-Me-C-TOM-CE Phosphoramidite

5-Me-C-TOM-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3064-025-Me-C-TOM-CE Phosphoramidite 0.25g £432.00 Quantity Add to Order
10-3064-905-Me-C-TOM-CE Phosphoramidite 100µmoles £173.00 Quantity Add to Order
10-3064-955-Me-C-TOM-CE Phosphoramidite 50µmoles £86.00 Quantity Add to Order

Description

5-Me-C-TOM-CE Phosphoramidite

Structure

Catalog Number: 10-3064-xx

Description: 5-Me-C-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-5-methyl-Cytidine,2'-O-triisopropylsilyloxymethyl-
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C52H74N5O10PSi M.W.: 988.25 F.W.: 319.21

Diluent: Anhydrous Acetonitrile
Coupling: See Technical Bulletin
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_RNA_TOM_Deprotection.pdf). Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

rSpacer is used to introduce an abasic site to an RNA sequence.

The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3064-02 0.25grams .25grams 2.53 71 42.6 26.63 19.36 14.2 3.55
10-3064-90 100µmoles .099grams 1 20 12 7.5 5.45 4 1
10-3064-95 50µmoles .049grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3064-02 0.25grams .25grams 3.78 .07 N/A N/A 14.42 N/A
10-3064-90 100µmoles .099grams 1.5 .07 N/A N/A 4.92 N/A
10-3064-95 50µmoles .049grams .75 .07 N/A N/A 1.79 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3064-02 0.25grams .25grams 3.78 .07 N/A 11.42 10.73

10-3064-90 100µmoles .099grams 1.5 .07 N/A 4.06 3.82

10-3064-95 50µmoles .049grams .75 .07 N/A 1.65 1.55

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200