| 5'8-Cyclo-dA CE Phosphoramidite |
Catalog Number: 10-1098-xx
Description: 8,5'-Cyclo-dA CE Phosphoramidite
5'-Dimethoxytrityl-N-benzoyl-8,5'-cyclo-2'-deoxyAdenosine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C47H50N7O7P |
M.W.: 855.92 |
F.W.: 311.19 |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling: 3 minute coupling time is recommended. Due to the steric hindrance at the 5' hydroxyl, the coupling time for the phosphoramidite immediately following the addition of the 8,5'-Cyclo-dA should be increased to 6 minutes. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
One of the major sources of DNA damage in all organisms is the UV component of sunlight. The predominant reaction induced by UV light on DNA is dimerization of adjacent pyrimidine bases leading to cyclobutane dimers (CPDs). The dimers formed in the most significant quantity are the cis-syn cyclobutane dimer of two thymine bases. Although formed routinely, these dimer products are efficiently excised and repaired enzymatically (nucleotide excision repair) or the dimerization is reversed by photolase enzymes. These lesions have been connected to the formation of squamous cell carcinomas. In addition, humans who lack ability to repair CPD lesions with high efficiency may be genetically predisposed to Xeroderma Pigmentosa (XP), a disease characterized by extreme sensitivity to sunlight and high frequency of skin cancer. Polymerases encountering unrepaired CPD lesions are quite error-prone, leading to incorrect base insertions and subsequent mutations.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200