| Asymmetric Doubler Phosphoramidite |
Catalog Number: 10-1921-xx
Description: Asymmetric Doubler Phosphoramidite
1-[5-(4,4'-dimethoxytrityloxy)pentylamido]-3-[5-fluorenomethoxycarbonyloxy
pentylamido]-propyl-2-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C57H69N4O10P |
M.W.: 1001.17 |
F.W.: 351.31 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Synthesis should be carried out on 1000 support. A 15 minute coupling time is recommended for the doubler addition. |
| Deprotection: - Complete the synthesis of the first branch, remove the final DMT, and terminate by capping for 30 min.- Remove the Fmoc group using 1M DBU in anhydrous acetonitrile for 5 minutes at room temperature.- Resume normal oligonucleotide synthesis to complete the second branch. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is supplied under license from ISIS Innovation Limited. |
Dendrimers
Dendrimers are discrete, highly branched, monodispersed polymers that possess patterns reminiscent of the branching of trees. Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons.1,2 Dendrimers offer the following advantages. Incorporation of label using γ-32P-ATP and polynucleotide kinase increases in proportion to the number of 5’-ends. Fluorescent signal also increases in proportion to the number of 5’-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the ‘bottom up’ approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target
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