Useful Information
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please get in touch with us:
email: logistics@cambio.co.uk or call+44
(0)1954 210 200.
Nucleic Acid Information
- Nucleic Acid Data
- 1 pmole of 1,000 bp RNA = 0.33 µg
- 1 pmole of 1,000 bp DNA = 0.66 µg (3.03 pmoles of ends)
- 1 pmole of pUC18/19 DNA (2,686 bp) = 1.77µg
- 1 pmole of lambda DNA (48,502 bp) = 32.01µg
Molecular Weight Conversions
- MW of a double-stranded DNA molecule = (# of base-pairs) x (650 daltons/basepair)
- MW of a single-stranded DNA molecule = (# of bases) x (330 daltons/base)
- MW of a single-stranded RNA molecule = (# of bases) x (340 daltons/base)
Estimating Tm of an Oligonucleotide
Tm = [4°C x # of Cs and Gs in the oligo] + [2°Cx # of As and
Ts in the oligo]
Estimating Molar Amounts of Primer
To estimate the weight of 1picomole of oligodeoxynucleotide, multiply the number
of nucleotides by 0.33ng.
For example, one picomole of a 24-mer is 7.92ng (24 x 0.33 ng = 7.92ng).
Estimating Molar Amounts of Template
To estimate the weight of 100fmoles of double-stranded DNA template, multiply the
number of kilobases by 66ng. For example, 100fmoles of a 2.7kb plasmid is 178ng
(2.7 x 66ng = 178ng).
|
Metric Prefixes
|
|
Factor
|
Prefix
|
Symbol
|
|
109
|
giga
|
G
|
|
106
|
mega
|
M
|
|
103
|
kilo
|
k
|
|
10-3
|
milli
|
m
|
|
10-6
|
micro
|
µ
|
|
10-9
|
nano
|
n
|
|
10-12
|
pico
|
p
|
|
10-15
|
femto
|
f
|
|
10-18
|
atto
|
a
|
|
10-21
|
zepto
|
z
|
Approximate Amount of Template (in nanograms) Required for DNA Sequencing Reactions
|
Metric Prefixes
|
Template
Required
(fmoles)
|
Template Size (insert + vector) in kilobases
|
|
|
1 kb
|
3 kb
|
5 kb
|
7 kb
|
9 kb
|
|
25
|
17
|
50
|
83
|
120
|
150
|
|
50
|
33
|
100
|
165
|
230
|
300
|
|
75
|
50
|
150
|
250
|
350
|
450
|
|
100
|
66
|
200
|
330
|
460
|
600
|
|
150
|
100
|
300
|
500
|
700
|
900
|
|
200
|
135
|
400
|
660
|
930
|
1200
|
|
250
|
165
|
500
|
830
|
1200
|
1550
|
|
300
|
200
|
600
|
1000
|
1400
|
1800
|
Protein Yield Information
Bacterial Cells: Escherichia coli or Salmonella typhimurium
|
Cell data
|
Per cell
|
Per litre at 109 cells per ml
|
|
Wet Weight
|
9.5 x 10–13g
|
0.95g
|
|
Dry Weight
|
2.8 x 10–13g
|
0.28g
|
|
Total Protein
|
1.55 x 10–13g
|
0.15g
|
|
Volume
|
1.15µm3=1 femtolitre
|
|
Protein conc. in the cell: 135mg/ml
|
Theoretical maximum yield for a protein of interest from 1 litre of culture (109
cells/ml):
0.1% of total protein: 150µg/litre
2.0% of total protein: 3mg/litre
50.0% of total protein: 75mg/litre