Application Note: Electrochemical probes
Thursday, 1 October 2020
Electrochemical probes are comprised of an oligonucleotide modified with a redox reporter on one terminus and an electrode surface at the other. Upon hybridization of a target to a redox reporter, a change in the electrochemical response of the reporter can be measured and used to detect the target. This format has been used for the detection of single-base mismatches, DNA methylation, important analytes, abasic sites, and DNA damage*.
* E. Palecek, M. Bartosik, Chem. Rev., 2012, 112, 3427-3481
- Background on electrochemically active labels
- Oligonucleotide modifications for electrochemical labeling
- Research application of aptamer-based biosensor development