Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure (Figure 1) with a weight of 66 kDa (2).
Lectins are, due to their specific binding to carbohydrate structures on the cell surface or elsewhere useful in haematology, immunology or as specific markers for membrane glycoprotein structures (2). Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. The lectin is blood group non-specific after neuraminidase treatment and agglutinates human erythrocytes at a concentration of ≥ 7,8 µg/ml.
A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the a-chain. The relative affinities of the lectin for galactose derivatives, as well as the structural basis of its T-antigen specificity, are explained by its protein structure (2).
Medicago’s Jacalin is supplied as a white lyophilized powder from a buffer containing 50 mM NH4HCO3, 10 µM CaCl2, no preservatives are added. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 12 kDa and 16 kDa. The lectin concentration is determined by amino acid analysis, minimum 80%. The material is made from one single batch; it is homogenous and is not derived from any material of animal origin. It is available in vials containing 100 mg or 10 mg lyophilized powder and is to be used for laboratory work only.
- Ultrapure quality
- Galactose-binding lectin
- Specific for a tumour-associated T-antigenic disaccharide
- Agglutinates human erythrocytes at a concentration of ≥ 7.8 µg/ml
- Lyophilized powder
Directions for use
The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.
Shipping and storage
The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
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