Transfection-EZ Biosystems: Broad Range Transfection Reagents
We offer a range of EZ Biosystems' general purpose transfection reagents for plasmid, siRNA, co-transfections, in vivo work and CRISPR. These reagents are cost-effective and have been specially formulated for maximum transfection efficiency of a wide range of different cell types with minimal toxicity.
Avalanche® transfection reagents have the broad spectrum for
the transfection of large plasmid, mRNA, siRNA, and/or other type of
nucleic acids. This feature makes Avalanche® transfection reagents the
best option for co-transfection of different type and/or size of nucleic
acids. The following are the reasons for the broad spectrum:
The
key components of Avalanche® transfection reagents are mixtures of
cationic lipids and cationic polymers, which contain all three types of
amines: primary, secondary, and tertiary amines. The three types of
amines have different positive charge strength, hence have different
capabilities to combine with negative charged anions.
As anions, different types and sizes of nucleic acids have different densities and quantities of negative charges.
With
optimized ratios of different cationic lipids and polymers, hence
optimized ratios of the three different type of amines, together with
optimized protocols, our Avalanche® mixtures have the optimized strength
of positive charges that ensure them to efficiently combine with
different type of nucleic acids of a variety of negative charge
densities and quantities, and compact them into the right sizes of
nanoparticles with the right densities of positive charge (zeta
potential) on their surface. The optimized size and the right strength
of zeta potential of the nanoparticles are the keys for the
nanoparticles to interact efficiently with negatively charged cell
surface, and trigger endocytosis process.
Avalanche-CRISPR Transfection Reagent
Avalanche-CRISPR Transfection Reagent
EZ Biosystems
Description
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CRISPR-Cas9–mediated genome targeting:
CRISPR-Cas9 has triggered a revolution in which laboratories around the world are using the technology for innovative applications in biology. This technology is able to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders.
The CRISPR-Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNA interference in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.
The CRISPR interference technique can be applied in eukaryotic cells. By delivering the Cas9 protein and appropriate guide RNAs (sgRNA) into a cell, the organism's genome can be cut at any desired location.
The Cas9 enzyme (blue) generates breaks in double-stranded DNA by using its two catalytic centers (blades) to cleave each strand of a DNA target site (gold) next to a PAM sequence (red) and matching the 20-nucleotide sequence (orange) of the single guide RNA (sgRNA).
The Transfection Reagent:
One of the most important factors that determines a successful CRISPR-Cas9–mediated genome targeting is the efficiency of delivering functional Cas9 gene and sgRNA into the cells (the transfection process). EZ Biosystems, the transfection and gene expression experts, has specifically developed the transfection reagent for CRISPR-Cas9 gene editing in mammalian cells ---- Avalanche®-CRISPR Transfection Reagent:
Features:
- Specifically designed for CRISPR-Cas9 gene editing transfection in mammalian cells
- Maximum efficiency for transfecting large plasmids (>9.0 kb)
- Great for co-transfection of large plasmids with either small plasmids or small RNA, such as sgRNA
- Great for CRISPR-Cas9 gene editing in difficult-to-transfect cells, such as primary cells, suspension culture cells, or neural cells.
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| Data: |
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| Avalanche®-CRISPR outperforms other brands of transfection reagents on transferring Cas9/GFP co-expression plasmids into some difficult-to-transfect cells. GFP expression in different type of cells after transfecting pCas9-GFP plasmid (Addgene #44719) by using Avalanche®-CRISPR, Lipofectamine® 2000 (L2K), and Lipofectamine® 3000 (L3K). The cells tested are: Human dermal fibroblast, BJAB (human Burkitt lymphoma cell), Human iPS cell, NSC-34 (Mouse Motor Neuron Hybrid Cell), C2C12 cell (mouse myoblast), and HUVEC (Human Umbilical Vein Endothelial Cell) |
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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Protocols
Download protocol here
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200