CoV Screen Kit
CoV Screen Kit is a broad RT-qPCR detection system for the RNA of the Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoV) that includes Minerva Biolabs's universal one-step RT-PCR enzyme mix ConviFlex™ RT-Taq Mix (Cat. No. 192-0025/192-0100/192-0250), which together allow qualitative detection of coronaviruses in extracted RNA samples.
Detection system for SARS-CoV viral RNA by RT-qPCR
CoV Screen is a broad RT-q PCR detection system for the RNA of the Severe Acute Respiratory Syndrome Coronaviruses (SARS-CoV). Combined with our universal one-step RT-PCR enzyme mix ConviFlex™ RT-Taq Mix (Cat. No. 192-0025/192-0100/192-0250), this primers/probes system allows qualitative detection of coronaviruses in extracted RNA samples.
This product is designed on the basis of the broad-spectrum SARS-CoV detection assays and sequences (both primers and probes) indicated bythe WHO reference diagnostic laboratory in Germany
(Ref. 1; https://www.who.int/emergencies/diseases/novel-coronavirus-2019/technical-guidance/laboratory-guidance). SARS-CoV-viruses are the causative agents of various infectious respiratory diseases.
Principle of the Method
CoV Screen kit contains lyophilized primers/probes, which amplify the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV, bat-SARS-related CoVs, and SARS-CoV-2 (also named 2019-nCoV). For the specific virus-targeting sequences of the primers and probe, please see Refs. 1, 2. A broad range of SARS-CoV viruses are detected by these primers/probes (Refs. 1, 2). Please note that this kit does therefore not discriminate between a general SARS-CoV virus and SARS-CoV-2. Specific detection of SARS-CoV-2 can be qualitatively achieved by RT-qPCR with our SARS-CoV-2 Confirm detection system (Cat. No. 171-02-0100)
RNA may be extracted from diverse starting materials like tissue, biopsies and swabs (including. respiratory specimens), mammalian cells and bacteria with a commercially-available RNA extraction kit (see ExtractNow™ Virus RNA Kit Cat. No. 611-1010/-1050) or a well-established extraction method.
The amplification of the SARS-CoV target (RdRp) is detected in the FAM™ channel. The mix includes also an amplification system for the human RNase P gene (process control), detected in the ROX™ channel, to assess the performance of the sample preparation procedure. Included is also a lyophilized Positive Control RNA containing a synthetic RNA sequence for the Wuhan-specific SARS-CoV-2 virus strain.
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