Catalog Number: 20-4040-xx
|5'-Dimethoxytrityl-N-trifluoroacetyl-puromycin,2'-succinoyl-long chain alkylamino-CPG
Diluent: Not Applicable
|Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
|Deprotection: Deprotect using ammonium hydroxide and the protocol required by the nucleobases.
|Storage: Controlled room temperature or lower, dry
|Stability in Solution: Not Applicable
One of the most challenging requirements associated with combinatorial chemistry is the recovery of sequence information of the oligonucleotide or peptide selected by the screening assay. A method1 has been developed to generate a fusion product between mRNA and the polypeptide it encodes using in vitro translation of synthetic RNAs 3’-labeled with puromycin, an antibiotic that mimics transfer RNA. Puromycin binds in the ribosome’s A site, forms a peptide bond with the growing peptide chain, and blocks further peptide elongation. By linking puromycin to mRNA, a peptide-RNA fusion product results from the translation of the message linking the encoding mRNA with its peptide product.
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